"Animals were weighed prior to initiation of the study, and on Day 1, 8, 15, 22, and 29. On Day 29, animals were euthanized by CO2 inhalation/asphyxiation.Organ weights were obtained for the liver, spleen, lungs, thymus, and kidneys."

Statistical Analyses: "All results, except the host resistance data, are presented as mean ± SE for 7–8 animals per group. All statistical analyses were conducted using SAS software (SAS Institute, Cary, NC). Jonckheere’s Test was used to test for dose-related trends. Data which typically exhibit a normal distribution were analyzed using a parametric multiple comparison procedure. If a significant trend was observed Williams’ test was used; if the trend was not significant Dunnett’s test was used. Positive control and vehicle control data were compared by Student’s t-test. Data for other endpoints were analyzed using a non-parametric multiple comparison procedure. If a significant trend was observed Shirley’s test was used; if the trend was not significant Dunn’s test was used. Positive control and vehicle control data were compared by a Wilcoxon Rank Sum Test. Host resistance data were analyzed by a Cochran–Armitage test, to establish single trend p values on binary moribundity data, and by a Fisher’s exact test to determine pairwise significance. Data that were different from control at p≥0.05 were considered statistically significant."

"Values represent the mean ± SE of all cohorts combined. Each cohort represented 8 animals per dose group. N = 11 cohorts for all dose groups."

Female Harlan Sprague Dawley Rat