"Mated rabbits were assigned to test groups daily in order to provide an equal distribution of mated females among groups and to equalize the GD 0 mean group body weights. Each group consisted of 20 females. Groups of rabbits were designated as either a control (0 ppm), 5000, 15,000, or 50,000 ppm HCFC-124."

"Four 6000-liter stainless steel and glass exposure chambers were used for the exposures. The chambers were operated in a one-pass, flow-through mode with the air flow rates of approximately 1200 liters/min. During exposure, the airflow rates, temperature, and relative humidity of the chamber air were recorded at approximately 30-min intervals. Exposures were conducted for 6 hr per day, during GD 6-18. The exposures were conducted during the same 8-hr period of the day to minimize diurnal physiological variations. Before transfer to housing facilities at the end of each exposure, test groups were left in their respective exposure chambers for a period sufficient to allow clearance of test material from the chamber atmosphere as determined by atmospheric analysis."

"The test material was introduced into the chamber air supply as a vapor. It was mixed with incoming conditioned air by turbulence. Chamber concentrations of HCFC-124 were controlled by varying the vapor flow rates into the exposure chamber. Conditioned air alone was metered in an identical manner into the respective control chambers. To maintain the cylinders containing the test material at a constant temperature, they were warmed to offset the temperature decrease due to volatilization of the liquid material. Chamber atmospheres were analyzed by gas chromatography at approximately 30-min intervals during each 6-hr exposure period. Samples were drawn by vacuum pump from representative areas of the chambers where the animals were exposed."

"Gas samples were analyzed with a gas chromatograph equipped with a flame ionization detector. Nitrogen was utilized as the carrier gas. Samples were chromatographed isothermally on stainless steel columns packed with either 2% OV-101 on 100/120 mesh Chromosorb WHP or Porapak Q 100/120 mesh. The atmospheric concentration of HCFC-124 was determined by comparing the detector response of chamber samples to that of gas standards through the use of standard curves. The gas standards were prepared by injecting known volumes of HCFC-124 vapor into gas bags which contained known volumes of air."

F1 Male and Female New Zealand White Rabbits