"Test material delivery: The test material was placed in a 50 milliliter (ml) glass syringe mounted on 1 Sage syringe pump (Model #352) for the low exposure (Group II). For the high exposure(Group III), approximately 1600 mls of PFBE was placed in a 2000 ml flask connected to an FMI Lab Pump(Model G-20), From these reservoirs, the test material was metered to a 4-neck roundbottom flask that was maintained at a temperature of 45°C using a water bath. Housellne dry air, at a flow rate of 7.0 liters per minute (lpm), was directed into the flask. The resultant vapour-laden airstream was delivered Into the exposure chamber.

Chamber operation: The animals were exposed in a glass chamber with a volume of 19.7 litres. The average air flow rate through the chamber was 7.0 l/min. This flow was calculated to provide one complete air change every 2.8 minutes and a 99% equilibrium time of 12.9 minutes.

Chamber concentrations were determined in the chambers, including control, every half hour using a GOW-MAC Model #750 gas chromatograph. A six foot 1/8" chromosorb 102 column

at a temperature of 160°C was employed for the chromatographic separation and determination. Chamber concentrations were determined by comparing the integrated GC responses obtained with primary gas standards. The GC sampling sequence was Groups I, II, III, I etc. However, because of the carry-over of PFBE in the sampling lines from high dose to control, this sequence was reversed on Day 2 and on Day 6 the sampling time was increased from 8 to 9 minutes.

Chamber oxygen was monitored daily (4 times per day for the first two days and twice daily thereafter) using an MSQ Portable Oxygen Indicatory (Type E).

Chamber air temperatures were recorded hourly during the exposures.

Chamber oxygen was monitored daily (4 times per day for the Females selected for mating were placed with male rats nightly in a 1:1 ratio. Vaginal smears were taken early in the morning and females were considered mated if sperm were observed microscopically in the vaginal rinse. The day when evidence of mating was observed was designated Day 1 of gestation.

Mated females were exposed daily for 6 hours/day from Day 6 to Day 15 of gestation.

Duration of test: Day 6 to Day 15 of gestation represented a 10 day treatment period.

No. of animals per sex per dose: 7 pregnant females"

P0 Female Sprague-Dawley Rat