"TEST ANIMALS

- Source: Charles River Laboratories, Inc. (Raleigh, N.C.)

- Females (if applicable) nulliparous and non-pregnant: Yes

- Age at study initiation: Approximately 51 days old

- Weight at study initiation: Males:

- Fasting period before study: All males and recovery phase females were fasted prior to clinical pathology blood collection when food, but not water, was withheld.

- Housing: Following receipt and until pairing, all F0 animals were housed 2–3/cage by sex in clean, solid-bottom cages with bedding material (Bed-O’Cobs; The Andersons, Cob Products Division, Maumee, OH). During cohabitation, breeding phase rats (10/sex/group) were paired in a solid-bottom cage with bedding material. Following the breeding period, the males were individually housed in solid-bottom cages until the scheduled necropsy. Following positive evidence of mating, the females were individually housed in solid-bottom cages with bedding material. The dams and their litters were housed in these cages until euthanasia on Lactation Day 13. Females that failed to deliver were housed in solid-bottom cages with bedding material until post-mating day 25. The 5 rats/sex in the control and high-exposure groups that were assigned to the recovery phase were not paired for mating and remained housed in groups of 2–3 in clean solid-bottom cages until euthanasia.

- Diet: PMI Nutrition International, LLC Certified Rodent LabDiet 5002 ad libitum.

- Water (e.g. ad libitum): Reverse osmosis-purified tap water ad libitum.

- Acclimation period: 21 days

ENVIRONMENTAL CONDITIONS

- Temperature (°C): 22.5-24.3 C

- Humidity (%): 30.3-62.6

- Air changes (per hr): At least 10

- Photoperiod (hrs dark / hrs light): 12/12"

Details on mating procedure:

- M/F ratio per cage: 1:1

- Length of cohabitation: 14 days

- Proof of pregnancy: vaginal plug or sperm in vaginal smear referred to as day 0 of pregnancy

- After 14 days of unsuccessful pairing, the pair was separated.

- After successful mating each pregnant female was caged (how): Individually."

P0 Male Sprague Dawley Rat