lactation index = "Number of live pups on lactation day 21/number of live pups on day 4 post-culling."

"The number of live and dead pups and clinical observations for the pups were recorded once daily during the postpartum period, and pup body weights were collected on LD 0, 4, 7, 14, and 21. On LD 4, litters were randomly reduced to eight pups each (four per sex when possible)...For the 1, 5, and 25 mg/kg/day groups, offspring in the F1 litters of each treatment level were randomly selected (one animal/sex/litter when possible) to be evaluated for collection of developmental landmark data. For the 100 mg/kg/day group, all offspring were euthanized as weanlings (PND 21) due to concerns about their ability to survive without the dam, based on low pup body weights and high pup mortality. Clinical observations, body weights, and food consumption data were collected on all surviving F1 generation mice weekly from PND 21 until PND 40–43. The age and body weight at either vaginal opening or preputial separation were recorded."

"Statistical analyses: Litter survival and indices for mating, fertility, and gestation were compared using Fisher’s Exact test [26] with a Bonferroni–Holm correction [27]. For all other continuous numerical data, transformations were applied when appropriate, based on preliminary tests for homogeneity of variance [28] and normality [29]; and a one-way analysis of variance [30] followed by Dunnett’s test [31] were conducted. For all statistical analyses, significance was judged at p < 0.05."

Note: Number of litters was entered for N because total number of pups alive on day 4 was not reported

F1 Male and Female Crl:CD1(ICR) Mice