"Exposure apparatus: NYU type stainless steel, Teflon, or glass; an NYU type 750L chamber was used for test substance exposures and an NYU type 1000L chamber was used for control (air only) exposures The exposure chambers were cubical with square-pyramidal chamber inlets and outlets with a tangential feed at the chamber inlet which promotes uniform chamber distribution of the test substance. The chamber volume was chosen so that the total body volume of the test animals was not expected to exceed approximately 5% of the chamber volume.

Source and rate of air: Houseline, filtered air

Method of conditioning air: Heated, round-bottom, flash evaporation flasks

System of generating vapour atmospheres: Flash evaporation of the test substance in air.

During each exposure, the atmospheric concentration of the test substance was determined at least hourly by gas chromatography. Samples of chamber atmosphere were drawn from the breathing zone of the animals and were directly injected into a gas chromatograph (GC) equipped with a gas sample valve and a flame ionization detector. All samples were chromatographed isothermally at 110°C on a fused silica glass column. The atmospheric concentration of the test substance was determined from a standard curve derived from gas standards. Standards were prepared prior to the exposure by injecting known volumes of the liquid test substance into Tedlar bags containing known volumes of air.

Duration of treatment/exposure: 107 days, approximately, for males; 121 days, approximately, for females

Frequency of treatment: 6 hours/day for 5 days/week during premating for approximately 10 weeks for males and females, 6 hours/day for 7 days/week as specified below:

Cohabitation period for males/females – for approximately 14 days

Postcohabitation for males – for approximately 23 days

When evidence of copulation was observed, the gestation period was initiated

Gestation for females – for approximately 20 days

Lactation for females – for approximately 17 days

Offspring were not exposed in the exposure chamber

Postcohabitation for females – for approximately 20 days

Females without evidence of copulation were exposed for 20 days or until evidence of delivery/impending delivery.

Doses / Concentrations: Males/Females: 0, 500, 2000, 3500 ppm (0; 5154; 20618; 36081 mg/m3, respectively)

Basis: nominal conc."

"The mean atmospheric concentrations over the entire exposure period (± standard error of the mean) were 500 ± 2.1, 2000 ± 2.9, and 3500 ± 5.6 ppm in chambers targeted at 500, 2000, and 3500 ppm, respectively."

P0 Female Crl:CD(SD) Rats