"Route of administration: inhalation: vapour Type of inhalation exposure: nose only Vehicle: clean air

Details on inhalation exposure: GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION

- Exposure apparatus: Aluminum or polypropylene inhalation chamber (46.7 L).

- Method of holding animals in test chamber: Animals were held in Battelle plastic animal holders in rodent tube sections with 20 exposure ports

- Source and rate of air: At least 1 L/min per animal.

- Temperature, humidity, pressure in air chamber: 29-25 C, 30-70% humidity

TEST ATMOSPHERE

- Samples taken from breathing zone: yes

Details on analytical verification of doses or concentrations: Test atmosphere concentration was measured by total carbon analysis (Sick Maihak EuroFID total carbon analyzer).

Duration of treatment / exposure: 6 hours a day

Frequency of treatment: Premating period: Male and female animals were exposed during 2 weeks prior to mating to the test substance for 5 days/week (10 exposure days in total). Mating period: Male and female rats were exposed daily. Gestation period: Female animals were exposed daily from mating (finding sperm positive vaginal smear; gestation day 0) up to (and including) gestation day 19. Females that appeared to be not pregnant were exposed daily during the same period. Male rats were exposed daily until sacrifice on 1 May 2015."

This study included a 750 ppm exposure group as well. "In the 750 ppm group, 11 females were places with males. Due to the unscheduled death of one male and one female during mating and the deteriorating conditions of the other animals in this group, exposure was discontinued and the animals were sacrificed after 9 days of cohabitation. The that time, 4 out of 11 females were sperm-positive and considered mated." There were no data reported for reproductive performance or for F1 pups in the 750 ppm group. Therefore, the 750 ppm group is not included in this extraction. Data for parental systemic toxicity for this group is extracted under ECHA 2016a_6385683.

P0 Male Wistar Rat