"Animals were dosed for 6 hours per day for the first 2 weeks for 5 days/week. Then the animals were exposed for 7 days/week during mating and female animals were exposed until gestation day 19. Male animals were exposed for 4 weeks and then sacrificed. Female animals were allowed to litter and were sacrificed at or shortly after lactation day 4.

GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION

- Exposure apparatus: The inhalation equipment was designed to expose rates to a continuous supply of fresh test atmosphere. Vapors of the test material for each test atmosphere were generated by passing a ow of approximately 5 L/min air through U-tube evaporators in which a ow fo test material was injected using a peristaltic pump (Watson and Marlow, type 502S). The evaporators were placed in a temperature controlled bath, held at 29C . The flows of air were diverted from the mass ow controlled and humidified mainstream for each exposure chamber and after passage through the evaporator reunited with the mainstream. The water container used form humification of the mainstream was / kept at a constant temperature of ca. 19.5 C. Each unit consists of a cylindrical column with a column of ca. 50 L, surrounded by a transparent hood. The test atmosphere was introduced at the top of the central column, and was exhausted at the bottom. For exposure to the animals of group B and C, each column included three rodent tube section of 20 ports each, for exposure of the animals of groups A and D four rodent tubes were necessary.

- Source and rate of air: 70, 51, 51 and 70 L/min for groups A, B, C and D

- Method of holding animals in test chamber: Tubes "

"Test atmosphere was analyzed using a Total Carbon Analyzer. Readings were taken every minute during the exposure periods. "

Doses calculated from ppm values noted in results section based on a molecular weight of 316.05 g/mol identified online using the CASRN reported. Doses identified from the results include: 300, 1,000, and 3,000 ppm exposure groups. The methods indicate groups A-D; these exposures combined with the control group appear to be the exposures used for this study.

P0 Male Wistar Rats