"Route of administration: inhalation: vapour

Type of inhalation exposure: whole body

Vehicle: other: air

Details on inhalation exposure: GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION

- Exposure apparatus: Three 750 L chambers (test substance exposure) and a 1000 L

chamber (control exposure). The chambers and all surfaces that the test substance

contacted prior to exhaust from the chamber were constructed of stainless steel and glass.

- Method of holding animals in test chamber: Two per cage in wire basket exposure

modules, except for approximately 2 weeks when rats were housed individually in wire

basket exposure modules.

- Source and rate of air: Not reported

- Method of conditioning air: Not reported

- System of generating particulates/aerosols: The chambers were operated in a one-pass,

flow-through mode. The test substance was generated by metering the test substance into

a glass J-tube filled with glass beads. Heated air was blown through the glass beads to

evaporate the liquid test substance and the resulting vapour was diluted to the desired

concentration with filtered air for each of the 3 test chambers. Chamber concentrations

were controlled by varying the test substance flow rates into the exposure chamber. Heated

air alone was metered in an identical manner into the control chamber.

- Temperature, humidity, pressure in air chamber: 19-24°C; 30-70%; pressure not reported

- Air 􀂡ow rate: 110-211 L/min (control chambers); 100-180 L/min (test chambers; except

test days 1 and 2, for males at 500 ppm where it was 72-73 L/min)

- Air change rate: Not reported

- Method of particle size determination: Not applicable

- Treatment of exhaust air: Diluted with room air and exhausted through a roof stack

TEST ATMOSPHERE

- Brief description of analytical method used: GC with a flame ionization detector; analyzed

at 30-minute intervals during each 6-hour exposure period. Diastereomer ratio (area percent)

from a test chamber was determined once daily (except test days 3, 29, 46, and 67). During

prestudy method development, distribution of test substance in the chamber was

determined.

- Samples taken from breathing zone: yes

Analytical verification of doses or concentrations: yes

Details on analytical verification of doses or concentrations:

Due to a spill of the test substance in the chamber room on test day 57, a small quantity of

test substance (8.5 ppm) was detected in the control chamber since the chambers were

operated under a negative pressure relative to the chamber room. However, the test

substance was only detected in 1 of the 10 samples collected, and the mean concentration

for the day was 0.8 ppm. For the 500 ppm chamber, mean daily concentrations ranged

between 475-530 ppm. Mean chamber concentration was lower (92% of nominal) than this

range on test day 58 and was higher (112% of nominal) than this range on test day 4. For the

2000 ppm chamber, mean daily concentrations ranged between 1800 and 2200 ppm. Mean

chamber concentration was lower (81-88% of nominal) on test days 3, 17, and 33. For the

3500 ppm chamber, mean daily concentration ranged between 3150-3600 ppm. Mean

chamber concentration was lower (87% of nominal) on test day 3.

Duration of treatment / exposure: 90 days (weekends and 1 holiday excluded)

Frequency of treatment: 6 hours/day, 5 days/week"

"Doses / concentrations

Doses / concentrations 1

Remarks: Doses / Concentrations:

0, 500, 2000, 3500 ppm (5154; 20618; 36081 mg/m3, respectively)

Basis:

nominal conc.

Doses / concentrations 2

Remarks: Doses / Concentrations:

0, 503, 1982, 3473 ppm (0; 5189; 20433; 35802 mg/m3, respectively)

Basis:

analytical conc."

Female Crl:CD®BR Rats