Animals were exposed by nose only inhalation.

"GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION

- Exposure apparatus: Nose-only, flow-past exposure using the Sachsse et al. (1973, 1976) method.

- Method of holding animals in test chamber: Restraint tubes

- Source and rate of air: Clean compressed air was utilized at a generator airflow of 10L/min and a dilution airflow rate of 6 L/min

- Method of conditioning air:

- System of generating particulates/aerosols: Test article vapor was generated using a nebulizer. The vapor was diluted with filtered compressed air to achieve the required exposure concentrations. The generated vapor was passed through a HEPA capsule filter to remove any aerosol droplets present.

- Treatment of exhaust air: No data

- Temperature, humidity, pressure in air chamber: Temp: 22.6-23.0 C, Humidity: 8.1-11.7 % relative humidity

TEST ATMOSPHERE

- Brief description of analytical method used: Gas chromatography

- Samples taken from breathing zone: yes"

"Deviations: Relative humidity of the room was 50-90%. N-hexane was used rather than dichloromethane to absorb the exposure atmosphere after finding incomplete absorption with dichloromethane. As a result, exposure of animals in group one with dichloromethane use were not reported. Only the results of the exposure of Group 2 utilizing n-hexane are contained in the report."

Note: Test concentration converted to mg/m3 by the extractor.

Female Wistar Rats