"GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION

- Exposure apparatus: Single Exposure Phase: 285-liter Lucite® chamber, Fitted with neoprene gloves for manipulation of the animals for FOB during exposure.

- Method of holding animals in test chamber:

Single Exposure Phase: 3 stainless steel wire cages, with 2 cages housing 4 rats and 1 cage housing 2 rats

- Source and rate of air: House air; rate not reported

- Method of conditioning air: Not reported

- System of generating particulates/aerosols: Atmospheres were generated by metering the liquid test substance using an infusion pump into heated round-bottom, three-neck, Instatherm flask. Generation air introduced at the 􀂡ask swept the resulting vapour through a

stainless steel distribution tube into the exposure chamber. Two battery operated fans helped to distribute the test substance evenly throughout the Lucite® exposure chamber for the single-exposure phase. House air alone was metered into the control chambers in an

identical manner. Each chamber operated in a one-pass, flow-through mode with air flow rates adequate to provide sufficient oxygen for the test rats, to prevent contamination from volatiles derived from excreta, and to enable adequate distribution of the test substance in the chamber.

- Temperature, humidity, pressure in air chamber:

Single Exposure Phase: 25-27°C; 29-49%; pressure not reported

- Air flow rate: Single Exposure Phase: 49-52 L/min

- Air change rate: Not reported

- Method of particle size determination: Not applicable

- Treatment of exhaust air: Chamber atmospheres were drawn from the bottom of the exposure chamber and exhausted into the fume hood.

TEST ATMOSPHERE

- Brief description of analytical method used: Chamber concentrations were determined at intervals at approximately 15-30 minutes during each exposure with a GC with flame ionization detector

- Samples taken from breathing zone: yes

Analytical concentrations were provided in ranges, Dose groups include the target concentration.

Male Crl:CD®BR Rats