"Route of administration: inhalation: vapour

Type of inhalation exposure: nose only

Vehicle: other: air

GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION

- Exposure apparatus: During the study with the 99.8% pure sample, the restrainers were inserted into the ports of a cylindrical, 0.9-L polycarbonate exposure chamber. During the study with the 99.9% pure sample, the restrainers were inserted into a faceplate attached to a 38-L glass exposure chamber.

- Exposure chamber volume: During the study with the 99.8% pure sample: 0.9-L polycarbonate exposure chamber. During the study with the 99.9% pure sample: 38-L glass exposure chamber.

- Method of holding animals in test chamber: Individually in polycarbonate cylinders with conical nose pieces.

- Source and rate of air: Not reported

- Method of conditioning air: Not reported

- System of generating particulates/aerosols: Test atmospheres were generated by metering the test substance into a three neck flask that was warmed in some cases to facilitate evaporation. High pressure air introduced at the flask swept the resulting vapour into the chamber. Supplementary oxygen was added to the chamber in the 2 highest concentration exposures to maintain adequate chamber oxygen.

- Method of particle size determination: Not reported

- Treatment of exhaust air: Test atmospheres were exhausted through carbon traps prior to discharge into the fume hood.

- Temperature, humidity, pressure in air chamber: Temperature was targeted at 23±2°C, humidity at 50±10%, pressure not reported, and oxygen at least 19%

TEST ATMOSPHERE

- Brief description of analytical method used: Atmospheric concentrations were determined using a GC. A GC _ame ionization detector and a stainless steel column packed with 2% OV- 101 were used in all but the highest concentration. At the highest concentration a thermal conductivity detector and a stainless steel column were used. Column temperatures were either 115 or 140°C. The chambers were sampled with a syringe in the 99.8% pure sample exposures and with an automatic gas sampling valve in the >99.9% pure sample exposures.

- Samples taken from breathing zone: Yes"

Male Crl:CD Br Rats