"Type of inhalation exposure: whole body

Vehicle: clean air

Details on inhalation exposure:

GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION

- Exposure apparatus: pictured, stainless steel exposure chamber but not further specified. Whole-body of animals was exposed to the test article as a vapour.

- Exposure chamber volume: 0.52 m³

- Source and rate of air: indoor air

- Method of conditioning air: filtered

- System of generating particulates/aerosols: test material was evaporated by bubbling in a heated atmosphere. Through two heat exchangers and flowmeter of the particles, the concentration of the test material was regulated.

- Treatment of exhaust air: charcoal filter

- Temperature, humidity, pressure in air chamber: 23 ± 2°C, 30

- 70%, between 0 and -15 mm Aq

TEST ATMOSPHERE

- Brief description of analytical method used: gas chromatography every 15 minutes

- Samples taken from breathing zone: yes Analytical verification of test atmosphere concentrations: yes

Remarks: GC

Duration of exposure: ca. 4 h

Concentrations: 3170, 3480, 3830, 4210, 4640, and 5100 ppm (males and females, nominal concentration)

3102.7 ± 15.6, 3475.4 ± 45.5, 3826.2 ± 28.4, 4223.6 ± 28.6, 4686.5 ± 159.4, and 5208.6 ± 145.4 ppm, respectively (analyzed concentrations)

19.04 ± 0.1, 21.33 ± 0.3, 23.48 ± 0.2, 25.92 ± 0.2, 28.77 ± 1.0, and 31.96 ± 0.9 mg/L, respectively (analyzed concentrations [in ppm] converted to vapour exposure units of mg/L)

No. of animals per sex per dose: 10

Control animals: no"

Note: Extractor converted exposure levels from mg/L to mg/m^3

Male CD Rats