"Type of inhalation exposure: nose only"

"Exposure apparatus: The rats were exposed nose-only to the test atmospheres. Animals were restrained in polycarbonate tubes supplied by Battelle, Geneva, Switzerland. These were inserted into a PERSPEX exposure chamber. The chamber was covered with an aluminium cone and stood on an aluminium base.

- Exposure chamber volume: The chamber consisted of sections of PERSPEX tubing (6 mm wall thickness) with an internal diameter of 28 cm and height of 15 cm (approximate volume 9.2 litres).

- System of generating vapour: The test atmosphere was generated using a heated, jacketed glass condenser. The test substance was pumped to the condenser using a Watson Marlow peristaltic pump. A counter current of clean, dry air (dried and filtered using equipment supplied by Atlas-Copco, Sweden) was passed at a nominal flow rate of 20I/minute (at normal temperature and pressure) through the condenser and carried the atmosphere to the exposure chamber (internal volume of 27.6 litres), in order to achieve a minimum of 12 air changes per hour. Since diluting air was not employed, the flow rates through the exposure chambers were the same as that employed in the generation of the test atmosphere. Air flows were monitored continuously and recorded at least 3 times using variable area flowmeters (KDG Flowmeters, Burgess Hill, Sussex, UK)

- Temperature, humidity, pressure in air chamber: The temperature and relative humidity in each chamber were recorded at least 3 times during exposure using a portable, digital temperature and relative humidity monitor; they were within the range of 20.4-21.2 °C and 15-20 % respectively.

TEST ATMOSPHERE

- Brief description of analytical method used: Samples were analysed using gas chromatography.

- Samples taken from breathing zone: yes. Test atmospheres were sampled from a front-facing port of the relevant exposure chamber, using a 5 ml gas-tight syringe equipped with an integral on-off valve and detachable sampling probe.

"Target concentration: 1800 ppm (correspond to 26.07 mg/I; MW=354.1355 g/mol) = maximum stable vapour concentration achievable, as determined in a trial generation.

Achieved concentration: 1761 ppm (correspond to 25.5 mg/I; MW= 354.1355 g/mol)" or 25506.45 mg/m3 (calculated by extractor)

Male and Female AlpK:APfsD (Wistar-derived) Rats