"GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION

- Exposure apparatus: The test atmosphere was generated using a large glass condenser

column and large round bottomed 􀂡ask. The test substance was delivered to the heated

glass column using a Watson Marlow pump (pump speed 4%). The pump tubing size was

817743. The glass condenser column was heated to approximately 50°C using a Techne

water bath.

- Exposure chamber volume: Approximately 9.2 L. The chamber located on to a base-plate,

fitted with castors for manoeuvrability. A conical aluminium lid ensured good distribution of

the atmosphere across the chamber, the atmosphere having being generated from above.

The conical lid and the base together had a volume of approximately 9.2 L. In this study two

sections were connected, giving a total chamber volume of approximately 27.6 L.

- Method of holding animals in test chamber: Animals were restrained in polycarbonate

tubes supplied by Battelle, Geneva, Switzerland

- Source and rate of air: Clean, dry air (dried and 􀂠ltered using equipment supplied by Atlas-

Copco, Sweden), at a 􀂡ow rate of 20 l/min

- Temperature, humidity, pressure in air chamber: 21.2-21.5ºC and 15-30%. Pressure has not

been measured.

TEST ATMOSPHERE

- Brief description of analytical method used: The test atmospheres were sampled manually

using gas tight syringes and analysed using gas chromatography. Each test atmosphere was

analysed regularly during each exposure period. The analysis system was calibrated using

an appropriate range of freshly prepared standards prior to the study and at intervals

thereafter. The peaks obtained were used to calculate the atmosphere concentrations.

Control atmospheres and room air were also sampled and analysed.

- Samples taken from breathing zone: yes

SELECTION OF TEST CONCENTRATIONS

- Rationale for the selection of the starting concentration: An atmospheric concentration of

1400 ppm (20 mg/L), a limit test concentration as recommended by a number of national

and international guidelines on toxicity testing was selected as the initial target exposure

level. A higher dose level was requested by the sponsor to generate safety data relevant to

human exposure.

- Duration of observation period following administration: 14 days

- Frequency of observations and weighing observations during exposure, immediately after

exposure and at the end of the observation period; weighing on day -1, 1, 8 and 15

- Necropsy of survivors performed: yes "

Male and Female Wistar Rat