"The organs and tissues were preserved in 10% neutral buffered formalin. Testes and epididymides were fixed in Bouin's solution.

Light microscopic examinations were performed for the following organs and tissues after embedding in paraffin, sectioning and hematoxylin and eosin staining. The histological preparation was conducted by Bio Pathology Institute, Ltd. according to the assignment protocol.

Livers were embedded in epoxy resin in the first 2 animals of the vehicle control, intermediate (1) and high dose groups for males and females, after double fixation by 2.0% glutaraldehyde in 0.1 M phosphate buffer and 1.0% osmium tetroxide in 0.1 M phosphate buffer. These specimens were not examined."

"Data regarding body weights, food intakes, hematological examinations, blood chemical examinations, urine volume and organ weights were analyzed using the Bartlett's test for homogeneity of variance. If the variances were homogeneous at a significance level of 5%, one way analysis of variance was performed. If there was a significant difference in this analysis, the difference between the vehicle control group and each of the treatment groups was analyzed by the Dunnett's test. If the variances were not homogeneous in the Kruskal-Wallis's test was used. If there was a significant difference in this analysis, the difference between the vehicle control group and each of the treatment group was analyzed by the nonparametric Dunnett's test."

Female Crj:CD(SD)IGS Rats