Don't use this study for pilot

Create 2 experiments and select reproductive study type for both. Both experiments will have P0, F1, and F2 animal groups.

Extract target concentrations (ppm) and also convert to mg/m3.

Mortality for each P0 group - use results text to extract quantitatively.

Clinical signs - create endpoint called "Clinical Observation Endpoints" for each animal group (except those only observed as fetuses on GD20) and use dummy variables (all 0) to indicate no treatment-related findings. Copy and paste results text into notes field.

Food consumption - P0 males and P0 females (maternal food consumption) of each experiment - use dummy variables to indicate no effect. Copy and paste results text into notes field.

Adult body weight change - use results text to extract dummy variables for each adult group evaluated (there was an effect on P0 male body weight change in the fertility study which should be indicated with dummy variable -1). Endpoints for pregnant or lactating females should be named as maternal body weight change. For fertility study, note that mean body weights are shown in Figure 1 (variances not reported).

Reproductive data

Note: In general, we extract the following types of endpoints under the maternal animals: corpora lutea, implantations, pre- and post-implantation loss, resorptions, intra-uterine death, litter size during gestation or at birth (e.g., total or live animals per litter), sex ratio during gestation or at birth, gestation length. In general, we extract the following under the offspring: fetal/pup body weight, litter weight, fetal/pup abnormalities, counts of live and dead offspring, postnatal survival (including litter size and sex ratio at timepoints after birth)

Tables 3-11 - extract all data except for table 8 only extract "Females with live young at weaning" (sample size is No. of females pregnant) for the main group.

Abnormalities

Table 12 (fertility study): only need to extract 2 endpoints: Fetuses with visceral defects, Fetuses with skeletal defects
Gross abnormalities: use the endpoint "External Malformations, Total" to extract dummy variables for each offspring group and timepoint that was evaluated in each experiment. Copy and paste results text into notes fields ("No abnormalities attributable to HFA-134a were detected in Fo and F1 males, in Fo and F1 females at termination of pregnancy or at the end of lactation, or in the sexually mature F2 animals. In addition, macroscopic examination of F1 and F2 generation rats at weaning did not reveal any abnormalities of significance."

F1 Combined AHA Rats