"All animals were observed daily for adverse clinical signs. Animals were weighed two to four times prior to being placed on test." " In study P-89, cytochrome P450 activity was measured in suspensions of liver microsomes (l to 2 mg protein/ml) using the method of Omura and Sato (1964). These determinations were made on liver samples that had been frozen at termination and stored on dry ice."."In A-77, a standard one-way analysis of variance was performed initially. If significant differences were noted, either Tukey's (all group sizes equal) or Scheffe's (group sizes different) test of multiple comparison was performed.

In D-78 all clinical chemistry data were compared by analysis of variance and least significant difference (LSD) tests for significance of difference (treated groups vs controls) when the ratio of variance indicated a compound-related effect. Significance was judged at p < 0.05. A one-factor analysis of variance was used to analyze absolute and relative organ and body weights. When the F-ratio analysis of variance was significant, the LSD test was used to compare treated groups to controls. When the F-ratio was not significant, treated and control groups were compared by Dunnett's test. In all cases, p < 0.05 was considered significant.

In P-89 and P-90, body weights, body weight gains, organ weights and clinical chemistry measurements were initially analyzed using the tests described for study D-78. Additionally, incidences of clinical observations were evaluated by Fisher's exact test with a Bonferroni correction. Hepatic peroxisomal ß-oxidation activity data were evaluated by Tukey's multiple comparison test. Bartlett's test for homogeneity of variances was performed on the clinical laboratory data. When the results of Bartlett's test were significant at p < 0.01 , the Kruskal—Wallis test was employed, and the Mann—Whitney U test was used to compare means from the control groups and each of the groups exposed to HCFC 123. Except for Bartlett's test, all other significance was judged at p < 0.05."

Male Sprague-Dawley Rats (P-90)