“All animals were regularly handled and examined daily for obvious changes or signs of reaction to treatment. Food consumption for F0 animals was measured weigh-day to weigh-day during the first 2 weeks of treatment (0-1, 2-3, 4-5, 6, 7, 8-9, 10-11, 12-13) and then weekly through to the end of the mating period. Thereafter, measurement for males continued on a weekly basis, and for females which gave birth measurement was reintroduced from weigh-day to weigh-day from Day I to Day 14 postpartum. For F, animals it was measured weekly. During the premating phase the food conversion ratios were calculated. Water consumption was measured on a daily basis during the first 2 weeks of treatment for both F0 and F, (postweaning) animals. All animals were weighed initially and on at least weekly intervals thereafter.

Litter Data At the end of the preweaning period (Day 21 postpartum), 12 male and 12 female pups in each group were retained for further study. Selection of pups was made using computer-generated random number tables using pups from all available litters. The total number of litters used in the selection for Groups I to 4 was 10, 8, 11 , and 11, respectively. On or shortly after Day 21 postpartum pups not selected for continuation were euthanized and examined externally and internally for abnormalities.”

F1 Sprague Dawley Rat