"The open field test (OF) was conducted after 8 weeks of chronic T0901317 treatment as described previously (Mulder et al., 2004) in a square, clear Plexiglas box (50 cm × 50 cm × 30 cm), with an open top and a grey floor. The movements and position of the animals were recorded and registered automatically by a computerized system (Etho VisionTM, Noldus, Wageningen, The Netherlands) in order to determine time in zone (TIZ) and distance moved (DM) over the 10 min trial."

"2.3.5. Statistics

All statistic analyses were performed using GraphPad Prism 4TM. The ORT and OLT were analyzed over time using the 2-way analysis of variances assay (2-way ANOVA) with time interval and treatment as independent variables. Animals that did not reach the minimum of 5 s exploration in T1 or T2 of the ORT or OLT were excluded from further analysis (Sik et al., 2003). d2-Values at the different time intervals were compared between the T0901317 and vehicle-fed group using an unpaired 2-sided Student T-test. In order to distinguish whether d2 values were different from 0, a one-sample Student T-test of data andA plaque quantification data were compared using an unpaired 2-sided Student T-test. When normality could not be proven due to a low power, mRNA and sterol levels were compared using the non-parametrical Mann–Whitney U-(MWU) test. The Kaplan Meier survival analysis was verified on significance using the log rank chi square test. Significance level were determined on P < 0.05, P < 0.01 or P < 0.001. In all data sets, extreme values were excluded by means of Dixon’s principles of exclusion of extreme values (Dixon, 1959a,b)."

Male C57BL/6NCrl Mice