Dichlorodifluoromethane is the only relevant chemical to extract for this study. Extract only the 90d study (referred to as Study C for continuous exposure) because the 6wk study does not have a concurrent control group to extract. You will create 5 animal groups for the 90d study representing the different species (sex of animals is not reported).

90-Day Inhalation (Study C) endpoints:

It is unclear if authors performed statistical analysis even though they refer to some changes as significant. Enter Not reported for all statistical test fields and use authors conclusions to set NOAEL/LOAEL for each endpoint.

Table 1 - Mortality for each species (control incidence in bottom row)

Clinical observation - use dummy variables and indicate no effects ("no visible signs of toxicity were noted in any species exposed to these materials")

Table 2 - extract white blood cells, hemoglobin, and hematocrit for each species. The table lists pre-exposure values (on top) and post-exposure values (on bottom). Only extract the post-exposure values. Control values are at the bottom of the table. Add to results notes "Although variations were noted in pre- and postexposure values of the hematologic components studied, none of these changes was considered significant" and "No significant hematologic alterations were noted in any of the studies"

Table 3 - extract body weight change (reported as %) for each species. "Body weight gains of the rabbits and guinea pigs were depressed" - use that statement to set the LOAELs.

*For histopathology endpoints, please copy descriptions of results from both studies into the results notes but we are using the results from the continuous exposure study to determine dummy variables and select NOAEL/LOAEL.

Lung histopathology, abnormal - use dummy variables and indicate increase for all species

Liver histopathology, abnormal - use dummy variables and indicate increase only for guinea pigs

Heart, spleen, kidney histopathology - use dummy variables and indicate no treatment-related changes for all species

Long-Evans or Sprague-Dawley Rats