"All the test chemicals were provided by Mond Division, Imperial Chemical Industries PLC, Runcorn, UK and were of commercial quality (99.5% pure). Their chemical formulas and FC-code numbers are recorded in Tables I and 2."

"Three-week-old, specific pathogen-free Alpk/Ap (Wistar derived) male and female rats were randomized and allocated to eight groups. The groups consisted of one un-dosed control, two vehicle dosed controls (one kept in a separate room), and treated groups dosed with FC22, FC3 I, FC 133a, FC 134a, and FC143a, respectively. Each group contained 36 male and 36 female animals except the un-dosed control which contained only 32 animals of each sex, and one dosed control group which contained 40 animals of each sex."

"The dosing solutions were prepared by dissolving the test compounds in Kraft Wesson 100% corn oil (Kraft Foods Limited, Liverpool, UK) to give a 3% w/v solution. (This was the maximum solubility which was practically obtainable due to the volatile nature of the materials.) The solutions were stored in sealed canisters pressurized to 10 psi with nitrogen. The appropriate dosing solution was drawn off into syringes via the fitted valves and after withdrawal the canisters were repressurized. Canisters containing the test solutions were stored at room temperature except FC143a which was stored at 4°C because of its exceptionally high volatility.

The dosing solutions were administered by gavage 5 days/week with a 15-gauge Portex nelaton catheter until week 12 of the study. Thereafter a 16-gauge, 7.6-cm-long stainless-steel cannula was used (Pertektum, Popper and Sons Limited, N.Y.). Animals were dosed at 300 mg/kg body weight with the 3% solution, i.e., 1 ml/100 g body weight. Dosed control groups were given 1 ml/l00 g body weight corn oil only. Dosing was continued until week 52 of the study, and the study was terminated at week 125."

Dose was adjusted by data extractor to account for discontinuous dosing: To obtain dose in mg/kg-day, the reported dose of 300 mg/kg BW was multiplied by a factor of 5/7 to account for dosing 5d/wk.

Male Alpk/Ap (Wistar derived) Rats