"During the treatment phase, the treated rats of all 10 study groups were placed on a diet consisting of the ground feed fortified with sulfluramid at a concentration of 75 mg of sulfluramid/kg of feed. The control rats were fed nonfortified, ground feed."

"Fresh feed was fortified every 2-3 weeks to avoid any possibility of a significant decrease in feed concentration of sulfluramid. The feed was fortified by adding 1.5 g of sulfluramid in 150 mL of acetone to 20 kg of ground rodent feed while blending thoroughly for 13 min in a mixer. The acetone was allowed to evaporate from the feed for 24 h prior to feeding. To verify the homogeneity, stability, and final concentration of sulfluramid, the feed was assayed weekly. Five randomly collected 2-g samples of the fortified feed were placed into 50-mL culture tubes with Teflon-lined screw caps. After 10 mL of Optima GC grade ethyl acetate (Fisher Scientific, West Haven, CT) was added to each sample, the tubes were vortexed and then centrifuged at HOOg for 30 min. The concentration of sulfluramid was quantitated by gas chromatography as described by Arrendale et al. (1989b)."

"The mean assayed concentration (71.0 ± 0.8 mg/kg of diet) of sulfluramid was about 5% below the amount added but appeared to be stable during the 2- or 3-week period of feeding the diet and between batches of diet (Table I). The low coefficient of variation for the assay results indicated homogeneity of distribution of sulfluramid in the diet."

"The mean daily sulfluramid consumption was 6.6 ± 0.5 mg/kg during the initial rapid growth period of the rats, which decreased to 4.9 ± 0.5 mg/kg as the rats matured."

"The mean daily consumption for the 56-day dosing period was 5.7 ± 0.6 mg/kg."

Clinical Observation Endpoints

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